Aims: Cancer is major health problem and cancer depending deaths have been increasing all over the world. Therefore, different treatment and management strategies such as phytotherapy have been used. Although there are important advances for the management and treatment of the disease, the cancer depending deaths have been increasing, too. So natural therapeutic agents obtained from different medical and aromatic plants such as capsaicin are used for the treatment of the disease. Because it is discussed that Capsaisin is a cancer predisposing factor besides its protective effect against cancer. The capsaicin is a natural therapeutic agents and has antiangiogenic activity, antimutagenic and antigenotoxic effects. It is discussed that Capsaisin is a cancer predisposing factor besides its protective effect against cancer. There is no study about the relation between AgNOR protein staining intensity and cultured human Caco-2 cell line with 25uM capsaicin application. So, we have performed the current study.

Methods: The Caco-2 cell line cultered and applied with 25uM capsaicin. The AgNOR staining method was performed on cultured human Caco-2 cell line without and with 25uM capsaicin application. Then staining intensity of AgNOR proteins using Gray Color Index (GCI) was detected. Results: The GCI values of cultured Caco-2 cell line was significantly lower than cultured Caco-2 with 25uM capsaicin application (p=0.000).

Conclusion: Capsaicin has potentially phytotherapeutic agents for human colon adenocarcinoma and AgNOR staining intensity may be used as indirect indicators to obtain knowledge about anticancer effects of the different phytotherapeutic agents such as capsaicin.

Keywords: AgNORs, Capsaicin, Caco2, Human colon adenocarcinoma, Staining intensity